STREOID'S COMPOUND INSULATION

STREOID'S COMPOUND INSULATION OF LIMAN'S TREAD LEAF

As much 4 sample gram are brayed with chloroform, then fitratnya is taken and is inserted into dropping plate and its dissolving letting to yawn, then is added 2 3 anhidrat's acetate drop and is swirled until all menjadin's residuums leaches then is added 1 2 sulphates sour drops stiff. Its appearance is colour green until blue menunjukan marks sense steroid. As compare utilized by kolosterol's powder 0,1 mg that ditetesi with pereaksi Lieberman burchard.

Steroid extraction

Dry sample as much 500 disokletasi's grams by use of dissolving n heksana. Sokletasi with heksana is discontinued if dissolving n unclouded heksana back and gotten by extract n heksana. Extract n heksana is gotten then tested by steroid by use of pereaksi Lieberman Buchard. Result tests positive drawned out by kromatografi endues and kromatografi is column by use of eluen would be convenient.

 

TLC

Kromotografi is lamella is done to extract stiff n heksana and fraction – fraction on kromatografi column by use of plate silica 60 GF254. Eluen who is utilized is n heksana, chloroform, and a variety compare of n heksana with Acetic ethyl. Ditotalkan's stiff extract intermediated by bottom bounds of plate by use of capillary tube and let by mengering is aired. Eluen already being made ready is inserted into camber and dijenuhkan by use of filter paper. Then plate is inserted into camber, then camber is closed. After eluen reaches plate upper limit, therefore appointed plate and let by mengering. Performance stainedding to utilize ultraviolet's lamp, pereaksi Lieberman burchhard and I2's yawn. That trick is done again and again by use of eluen is different, so on eluen one particular acquired blot which is diqoined with every consideration and further is utilized as eluen for kromatografi column.

 

Kromogtografi is column

Kromatografi's column that will be utilized washed beforehand with water until quite a clear, then is dried at deep oven, column afterwards kromatografi is flushed with heksana. As absorb as (silent phase) utilized by silica gel, one that beforehand being made as pap with dissolving n heksana. kromotografi's column is nipped with clamp on course vertical dank ran kromotografi's column is closed. Dissolving n heksana is inserted until one third kromotografi's column. Hereafter cotton or glass is wool already being soaked is inserted into kromotografi, then is compacted until air bubble can't come in again into kromotografi. silica's pap gel already being made is inserted into kromotografi's column cautiously so has no again air bubble. Upon silica's pap inserted by kromotografi dibiarakan's column faucet is opened, pap afterwards silica is compacted in kromotografi's column by overlooks dissolving over and over. Sample who will come to pieces to do pre beforehand imbibition, by dissolves it with heksana and added by silica gel, dissolving afterwards it letting to yawn. Sample is inserted into kromotografi's column then dielusi by use of fasa power n heksana and alae acetic ethyl elusi gets gradient (step gardien polarity). Kromotografi's result column is kept all at deep phial which contains 10 ml and given by ordinals. To monitor kromogtografi's result this column did by kromogtografi lamella and blot performance can be done by ultraviolet's lamp, Pereaksi Lieberman Burchard and I2's yawn. Of kromotografi's quiz lamella that memberkan Rf's price (esensi's factor)same, then is merged as one fraction and its dissolving is evaporated.

 

Rekristalisasi

Rekristalisasi did by methanol. On methanol's enhanced Crystal then is heated, so crystal and its pollutant leach. Then is idled on room temperature. After most crystal back therefore crystal and filtratnya is came to pieces. Hereafter Crystal dissolved by acetic ethyl and is let until its dissolving yawns. So will crystallized needle.